herpes simplex expression

Herpes Simplex Virus Gene Expression

Expression of the approximately 70 herpes simplex virus type 1 (HSV-1) genes is regulated primarily at the level of mRNA transcription, and can be conveniently divided into three phases based on the t...

A Modular Set of Helper-Dependent Herpes Simplex …

 · Introduction Herpes simplex virus (HSV) has many favorable properties in terms of its potential to serve as a delivery and expression platform for gene-based therapies (1, 2, 3, 4) rst, the virus has evolved a variety of immunoevasion strategies which it uses to ...

Variations in Herpes Infection Symptoms May Be Linked to …

 · A new study finds that differences in the symptoms of herpes could be due to variations in the way certain strains of herpes simplex (HSV-1) – the virus that causes cold sores, as well as genital herpes – activate gene expression in neurons.

Cellular Antisilencing Elements Support Transgene …

 · Although herpes simplex virus (HSV) vectors have the capacity to meet this need, the challenge has been to genetically engineer the HSV genome in a manner to prevent expression of any viral genes while retaining the ability to express multiple therapeutic

Regulation of herpes simplex virus gene expression

 · Expression of the more than 80 individual genes of herpes simplex virus 1 (HSV-1) takes place in a tightly regulated sequential manner that was first described over 20 years ago. Investigations since that time have focused on understanding the mechanisms that regulate this orderly and efficient expression of viral genes.

MicroRNAs expressed by herpes simplex virus 1 during …

 · A LAT-associated function reduces productive-cycle gene expression during acute infection of murine sensory neurons with herpes simplex virus type 1. J. Virol. 71, 5885–5893 (1997)

Herpes simplex virus gene expression in neurons: viral DNA …

Herpes simplex virus establishes a latent infection in peripheral neurons. We examined viral gene expression in rat peripheral neurons in vitro and determined that viral gene expression is attenuated and delayed in these neurons compared with that in Vero cells.

Herpes simplex virus

 · Abstract Latency-associated transcript (LAT) sequences regulate herpes simplex virus (HSV) latency and reactivation from sensory neurons. We found a HSV-2 LAT-related microRNA (miRNA) designated miR-I in transfected and infected cells in vitro and in acutely and latently infected ganglia of guinea pigs in vivo. miR-I is also expressed in human sacral dorsal root ganglia latently infected with ...

Expression, purification of herpes simplex virus type 1 US11 …

 · Pan W, Ren X, Guo H, Ding Q, Zheng AC: Expression, purification of herpes simplex virus type 1 UL4 protein, and production and characterization of UL4 polyclonal antibody. J Virol Methods 2010, 163: 465-469. 10.1016/j.jviromet.2009.11.015 Article CAS

De Novo Herpes Simplex Virus VP16 Expression Gates a …

 · De Novo Herpes Simplex Virus VP16 Expression Gates a Dynamic Programmatic Transition and Sets the Latent/Lytic Balance during Acute Infection in Trigeminal Ganglia. Sawtell NM(1), Thompson RL(2). Author information: (1)Department of Pediatrics, Division of Infectious Diseases, Cincinnati Children''s Hospital Medical Center, Cincinnati, Ohio, United States of America.

Regulation of herpes simplex virus gene expression

Expression of the more than 80 individual genes of herpes simplex virus 1 (HSV-1) takes place in a tightly regulated sequential manner that was first described over 20 years ago. Investigations since that time have focused on understanding the mechanisms that regulate this …

Optimized Expression, Purification of Herpes B Virus gD …

 · Protein Purification Monoclonal Antibody Herpes B Virus (BV) gD Protein Optimized Expression 1. Background Herpesviridae is a family of viruses with double-stranded DNA. The B virus (Cercopithecine herpesvirus 1, or herpes B virus) and Cercopithecine herpesvirus 2 (CeHV-2) are primate herpes viruses that belong to the alpha-herpesvirus subfamily, and are closely related to herpes simplex …

Regulation of Expression ofthe Herpes Simplex Virus DNA Polymerase

During herpes simplex virus infection, expression of the viral DNApolymerase (pol) gene is regulated temporally as an early (p) gene and is additionally down-regulated at late times at the level ...

Inhibition of herpes simplex virus 1 gene expression and …

How to cite this article: Liu, J. et al. Inhibition of herpes simplex virus 1 gene expression and replication by RNase P-associated external guide sequences. Sci. Rep. 6, 27068; doi: 10.1038 ...

[PDF] A Herpes Simplex Virus Vector System for …

ABSTRACT Viral vector-based gene expression libraries from normal or diseased tissues offer opportunities to interrogate cellular functions that influence or participate directly in specific biological processes. Here we report the creation and characterization of a herpes simplex virus (HSV)-based expression library consisting of cDNAs derived from PC12 pheochromocytoma cells. A replication ...

Differences in herpes virus symptoms may relate to …

 · Oct. 26, 2017 — Four in five adults are infected with herpes simplex virus, say researchers, but most don''t show symptoms like cold sores because the virus infection is ''latent'' -- sleeping ...

Expression, purification of herpes simplex virus type 1 …

Pan W, Ren X, Guo H, Ding Q, Zheng AC: Expression, purification of herpes simplex virus type 1 UL4 protein, and production and characterization of UL4 polyclonal antibody. J Virol Methods 2010, 163: 465-469. 10.1016/j.jviromet.2009.11.015 Article CAS

Herpes Simplex Virus (HSV) Vectors

 · Herpes Simplex Virus (HSV) Vectors Recombinant herpes simplex virus (HSV) has recently emerged as a promising viral vector in gene therapy and cancer therapy, with a variety of applications including gene delivery into the nervous system, as oncolytic agents, and for the development of vaccines against cancer, HSV and other infectious diseases.

Herpes Simplex Virus Vector

Fig.1 Herpes simplex virus vector genome Partial deletion of the HSV gene results in an expression vector with low toxicity and an excellent packaging capacity of >30 kb foreign DNA. Generally, the mutant strains of HSV-1 are used that lack the ability to replicate to avoid the host immune response.

An acutely and latently expressed herpes simplex virus 2 …

Abstract Latency-associated transcript (LAT) sequences regulate herpes simplex virus (HSV) latency and reactivation from sensory neurons. We found a HSV-2 LAT-related microRNA (miRNA) designated miR-I in transfected and infected cells in vitro and in acutely and latently infected ganglia of guinea pigs in vivo. miR-I is also expressed in human sacral dorsal root ganglia latently infected with ...

Expression of variable viruses as herpes simplex …

 · Expression of glycoprotein D of herpes simplex virus type 1 in a recombinant baculovirus: protective responses and T cell recognition of the recombinant-infected cell extracts J. Gen. Virol., 70 ( 7 ) ( 1989 ), pp. 1805 - 1814

De Novo Herpes Simplex Virus VP16 Expression Gates a …

 · Jurak I, Hackenberg M, Kim JY, Pesola JM, Everett RD, et al. (2014) Expression of herpes simplex virus 1 microRNAs in cell culture models of quiescent and latent infection. J Virol 88: 2337–2339. pmid:24307587 View Article 78.

Herpes simplex virus

Herpes simplex virus 1 and 2 (HSV-1 and HSV-2), also known by their taxonomical names Human alphaherpesvirus 1 and Human alphaherpesvirus 2, are two members of the human Herpesviridae family, a set of new viruses that produce viral infections in the majority of humans. Both HSV-1 (which produces most ...

Herpes simplex virus type 1 persists in the aged brain …

Herpes simplex virus type 1 gene expression and reactivation of latent infection in the central nervous system. Neuropathol Appl Neurobiol 20: 253–260. CAS Article PubMed Google Scholar Taddeo B, Zhang W, Roizman B (2006). The UL41 cleavage in absence ...

Inhibition of herpes simplex virus 1 gene expression and …

 · A decrease of about 97% and 75% in the level of ICP4 gene expression and an inhibition of about 7,000- and 500-fold in viral growth were observed in HSV infected cells expressing the variant and the pre-tRNA-derived EGS, respectively. This study shows that engineered EGSs can inhibit HSV-1 gene expression and viral growth.

Regulation of Expression ofthe Herpes Simplex Virus DNA …

During herpes simplex virus infection, expression of the viral DNApolymerase (pol) gene is regulated temporally as an early (p) gene and is additionally down-regulated at late times at the level ...